IMMUNOSEQ TECHNOLOGY

Accuracy and quantification by design

immunoSEQ Assays utilize a multiplex PCR-based assay enabling us to sequence directly from genomic DNA. We provide precise, quantitative abundance data on your cell populations, unlike the relative abundance or variable expression data of RNA-based assays. Each immunoSEQ assay contains synthetic immune templates as in-line controls that ensure accurate, quantitative, and unbiased results with batch-to-batch consistency.

First, a synthetic immune repertoire was designed to represent all VJ-gene combinations.

Then, measuring against our synthetic repertoire, we were able to adjust our primer concentrations to significantly reduce amplification bias.

In-line controls for every sample, enable us to measure amplification, correct residual bias & provide absolute counts of the immune cells in your sample.

Sophisticated bioinformatics correct errors & collapse sequences to deliver highly accurate, high-volume, standardized data sets.

Read the full story in Nature Communications:

“Using synthetic templates to design an unbiased multiplex PCR assay”

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immunoSEQ Assays are for Research Use Only. Not for use in diagnostic procedures.

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